Strand-specific detection of overlapping transcripts via purification involving denaturation of biotinylated cDNA
Method Summary
cDNAs synthesized with biotin-tagged primers were bound to streptavidin magnetic beads followed by alkaline denaturation to disrupt cDNA–cDNA duplex, potentially formed by sense and antisense overlapping cDNAs. Untagged cDNAs synthesized owing to self-priming were subsequently washed out, and biotin-tagged cDNA of interest was selectively enriched.
Authors: Faizan Uddin & Madhulika Srivastava.
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