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BioTechniques Vol. 70 No. 2 | Reports

Urea-based amino sugar agent clears murine liver and preserves protein fluorescence and lipophilic dyes

Method Summary

This study presents a simplified protocol for optically clearing murine liver tissue in only 24 h using one simple urea-based amino sugar solution and a single incubation. This method preserves fluorescence of transgenically expressed proteins and lipophilic tracer dyes within the context of native spatial morphology.

Authors: Hough M, Fenlon M, Glazier A et al.

View the full protocol on optical clearing here