Tips for Extracting DNA from Insects - BioTechniques

Tips for Extracting DNA from Insects

DNA sequencing

DNA

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Discover how a simple enzymatic method allows efficient extraction of DNA from insects while maximizing yields and minimizing waste.

Time:
1 April 2020, 10am EDT [3pm BST]

MicroGEM’s Rob Thompson will introduce a simple protocol for extracting DNA from insects using a single tube, without ionic detergents, magnetic beads, columns or centrifugation. He will use case studies featuring a range of insects including fruit flies, anopheles mosquitoes, and African whitefly, and will present qPCR data comparing enzymatic lysis with traditional spin-column methods.

Join us to discover the advantages of temperature-driven, enzymatic extractions that provide maximum DNA recovery, a simplified workflow, and significant reduction of plastic and chemical waste - all in minutes, not hours.

Learning outcomes include:
• The differences between chemical and enzymatic, heat-driven DNA extractions
• A method to extract DNA from small/scarce/challenging insect samples in less than 20 minutes
• Optimizing extractions for downstream applications including PCR, qPCR, whole genome amplification, and amplicon sequencing