An introduction to high-resolution DNA melting analysis
Methods for detecting sequence variants are essential for genotyping SNPs and mutational analysis of tumor cells. One of the simplest, fastest, and least costly approaches to detect DNA sequence variation is high-resolution melting analysis (HRMA).
In this special tutorial webinar, University of Utah researcher Carl Wittwer will discuss the fundamental principles behind HRMA. He will describe how to carry out HRMA for targeted genotyping using small amplicons, unlabeled probes, and snapback primers, as well as how HRMA can be applied in rapid genetic analysis and for copy number estimation.
University of Utah
Dr. Wittwer is a Professor of Pathology at the University of Utah Medical School. He has published more than 180 research articles and book chapters focusing on techniques and instrument development in molecular diagnostics.
He developed rapid-cycle PCR techniques for DNA amplification, and introduced SYBR Green I, fluorescent hybridization probes, and melting analysis to real-time PCR. His lab is now focused on developing high-resolution DNA melting analysis methods for variant genotyping and scanning.