How to sequence full-length 16S and ITS microbial amplicons

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What will you learn?

• How to prepare full-length 16S and ITS amplicons using the Microbial Amplicon Barcoding Kit 

• How to perform Oxford Nanopore sequencing for accurate taxonomic characterization of bacteria, archaea and fungi 

• How to use the EPI2ME™ workflow (wf-16S) to analyze sequencing data and interpret results 

• How real-time nanopore sequencing enables rapid, end-to-end microbial community profiling 

• Where to access supporting documentation, protocols and online resources for continued learning 

Who this may interest?

• Microbiologists studying bacterial, archaeal or fungal diversity in environmental or clinical samples 

• Researchers working in microbial ecology, metagenomics or applied microbiology 

• Quality and process scientists in biopharma or industrial microbiology, seeking fast microbial identification methods 

• Academics and educators looking to introduce full-length 16S and ITS sequencing into teaching or research programs 

• Sequencing facility and core lab staff evaluating end-to-end microbial workflows for streamlined analysis 

Speakers

Anna Maria Niewiadomska

Segment market manger – public health

Oxford Nanopore Technologies (UK)

In her role as Segment Market Manager, Anna drives the development of new markets in the infectious disease and public health space. She received her PhD in molecular microbiology and immunology from the Johns Hopkins Bloomberg School of Public Health (MD, USA). Prior to joining Oxford Nanopore, Anna worked on multiple projects focusing on host–virus interactions, mathematical modeling of antimicrobial resistance, microbial bioinformatics and emerging infectious disease outbreaks. She combines a deep knowledge of experimental and in silico methods for generation and analysis of microbial genomic data. 

Natalia Garcia

Bioinformatics software developer

Oxford Nanopore Technologies (UK)

As a Bioinformatics Workflow Developer in the Customer Workflow team, Natalia creates and maintains workflows that help non-expert users reliably analyze their data with minimal training. These open-source workflows, implemented using Nextflow, allow life science researchers to go from raw reads to informative reports designed to help them make the most of their data. In particular, she is focused on the analysis of microbial communities through metagenomic data. Before joining Oxford Nanopore in 2023, she worked on microbial ecology at the Microbiome Analysis Laboratory in the Spanish National Biotechnology Center (Madrid, Spain). 

Catriona Williams

Development scientist – Sample Technology

Oxford Nanopore Technologies (UK)

As a core member of the Sample Technology team, Catriona works on developing new sample and library preparation methods, sequencing kits and end-end-workflows. She worked on the development and release of the Ultra-Long DNA sequencing Kit and the Telomere-to-telomere (T2T) end-to-end workflow before focusing on the Microbial Amplicon Barcoding Kit. Prior to joining Oxford Nanopore in 2021, Catriona worked at Oxford BioDynamics (Oxford, UK) developing precision medical tests and at the UCL Institute for Liver & Digestive Health (London, UK).