Considerations for choosing a 16S rRNA gene library preparation protocol

Since the beginning of the microbiome era decades ago, 16S rRNA gene-based amplicon sequencing has been widely adopted for microbial composition profiling. Many researchers might assume the 16S sequencing workflow is now well-optimized and selecting a heavily-cited protocol is trivial; however, there are challenging aspects of 16S sequencing that should be considered before adopting a protocol.

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