Peek behind the paper: Site-specific analysis of ribosomal 2′O-methylation by quantitative reverse transcription PCR under low deoxynucleotide triphosphate concentrations

In this ‘Peek behind the paper’, Elena Martens-Uzunova (left), Assistant Professor in the Department of Urology at the Erasmus University Medical Center (Rotterdam, The Netherlands), discusses her paper on the development of a PCR technique that allows the quantitation of ribosome methylation (2’O-methylation) in small amounts of RNA from clinically obtained tumor tissue, which was recently published in BioTechniques. Martens-Uzunova's research focusses on prostate cancer – the most common cancer in men – investigating how molecular changes in noncoding RNA affect the survival and function of tumor cells and how these changes can be used to improve the treatment of...