Welcome to the 74th Issue of BioTechniques
In this issue of BioTechniques, we present a novel quantitative PCR-compatible assay designed to target intestinal microbiome markers, DNA extraction from frozen clotted blood, a new method for the preservation and metatranscriptomic analysis of small quantities of saliva, a technique for removing detergents from protein solubilized from inclusion bodies, and an overview of the methodology, principles, and applications of polyacrylamide gel electrophoresis.
Foreword
Welcome to the 74th Issue of BioTechniques: 40 years of publishing the latest laboratory protocols
Technology News
Ancient DNA sequencing: telling the tale of human history and evolution
Reports
Prediction of high fecal propionate-to-butyrate ratios using 16S rRNA-based detection of bacterial groups with liquid array diagnostics
DNA extraction from clotted blood in genotyping quality
A clinically validated human saliva metatranscriptomic test for global systems biology studies
Benchmark
Amberlite XAD-4 is a convenient tool for removing Triton X-100 and Sarkosyl from protein solutions
Review
Polyacrylamide gel electrophoresis: a versatile tool for the separation of nanoclusters
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