Long-read sequencing, metagenomics and the microbiome

Microbiology PCR and sequencing

In this episode, sponsored by Zymo Research, we explore the microbiome, how long-read sequencing techniques are changing our approach to the field and the exciting discoveries that they have led to. To do this we speak to Kris Locken, Molecular Biologist in the Research & Development department of Zymo Research (CA, USA), and Jeremy Wilkinson, Global Marketing Specialist for Microbial Genomics at PacBio (CA, USA).

Find out how microbes compare to nuts and what this means for the challenges of metagenomic sample preparation, how long reads can stack up vs short reads for metagenome assembly and much more, all in this latest episode of Talking Techniques

Contents:

  • Intro: 00:00-01:55
  • What is metagenomics and why is it important for microbial studies? 01:55-03:55
  • How has long-read sequencing impacted metagenomics? 03:55-06:05
  • Long-read vs short-read sequencing workflows: 06:05-07:15
  • Addressing barriers to long-read sequencing: 07:15-08:45
  • Sample preparation for long-read sequencing: 08:45-12:20
  • Development of long-read sequencing to improve accuracy and capabilities: 12:20-14:40
  • Best practice for assembly and analysis: 14:40-17:40
  • Exciting examples of the benefits of long-read sequencing: 17:40-20:00
  • What would you wish for to improve the ability long-read sequencing in microbiomics: 20:00-21:40

This podcast episode was produced in collaboration with Zymo Research.