Nanopore sequencing: how does it work?

Written by Oxford Nanopore

Oxford Nanopore  sequencing devices use flow cells that contain tiny holes – nanopores – embedded in an electro-resistant membrane. When DNA passes through a nanopore, the current is disrupted, producing a ‘squiggle’ that is specific to the particular base that has passed through in that moment. These squiggles can be decoded using basecalling algorithms to determine the DNA or RNA sequence in real time.

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