A single-cell multiomics approach to study tumor-driven perturbations during hematopoiesis in mice

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Cancer can interfere with normal hematopoiesis by skewing the generation of myeloid cells with tumor -promoting functions and inducing splenic extramedullary hematopoiesis. This webinar will showcase a single-cell multiomics approach for the investigation of alterations in hematopoietic stem and progenitor cell (HSPC) populations in a melanoma mouse model. First, we will describe an optimal workflow for sample processing and sorting of four rare HSPC populations. We will then describe the downstream single-cell multiomics analysis of isolated cells for the simultaneous assessment of surface protein and whole-transcriptome expression. We will also highlight the use of high-dimensional data analysis tools for deep cellular characterization to uncover tumor-mediated abnormalities in hematopoiesis.

What will you learn?

  • Best practices for cell preparation and sorting of rare cell populations
  • Strategies to increase sample throughput by combining multiple samples in an integrated workflow
  • Utilizing high-dimensional data analysis tools for simultaneous analysis of surface protein and whole-transcriptome expression at the single-cell level to enable deep cellular characterization and heterogeneity resolution

Who may this interest?

  • Core facility directors and research investigators engaged in basic immunology, stem cell biology, immune monitoring, and immuno-oncology studies
  • Researchers interested in the latest cutting-edge high-parameter data and associated analysis
  • Anyone interested in learning more about single-cell multiomics

Speaker

Nihan Kara
Senior Scientist, Immunology Applications, Medical and Scientific Affairs
BD Biosciences (CA, USA)

Nihan Kara is a Senior Scientist in Medical and Scientific Affairs at BD Biosciences, where she is involved in developing a variety of flow cytometry and cell-based assays for innovative immunology applications. During her graduate studies, she focused on studying the mechanism and control of DNA replication at Cold Spring Harbor Laboratory (NY, USA) and received her PhD in molecular and cellular biology from Stony Brook University (NY, USA). She has made important scientific contributions in deciphering the in vivo dynamics of DNA replication initiation and elucidating the mechanism of immunodeficiency syndromes.