Peek Behind the Paper: Comparing injection methods for high-throughput RNA silencing using CRISPR-rfxCas13d

In this Peek Behind the Paper, we learn more about a recently published paper by Joaquin Abugattas-Nuñez Del Prado (Leiden University, The Netherlands; Universidad Peruana Cayetano Heredia, Lima, Perú) and Herman Spaink (Leiden University). The pair worked together to compare robotic and manual injection methods in zebrafish embryos using CRISPR-RfxCas13d (CasRx) for RNA silencing. Joaquin Abugattas-Nuñez Del Prado (left) is a PhD candidate whose work focuses on investigating angiogenesis and its cellular, molecular and mechanical interactions with the extracellular matrix. He employs the intersegmental vessel model in the zebrafish, coupled with high-resolution microscopy and cutting-edge CRISPR-CasRx systems for transcriptomic modifications,...