Become a member of BioTechniques (it's free!) and receive the latest news in the life sciences, member-exclusives and 10% off BioTechniques article processing fees

Antibody validation for successful immunohistochemistry and multiplex immunofluorescence


Advances in cancer treatment using immunotherapies has accelerated the need to examine the tumor immune microenvironment (TIME). Multiplex immunofluorescence (mIF) assays allow the researcher to examine the spatial biology of tissues to determine tumor and immune cell interactions. The successful application of mIF requires highly validated immunohistochemistry antibodies in order facilitate mIF assay optimization.

This webinar describes how to evaluate and select the correct monoclonal antibody for use in mIF assays and introduces a simplified approach to optimize antibodies for use in an immune checkpoint multiplex assay, resulting in half the time typically spent on validating multiplex panels.

What will you learn?

  • Quality issues surrounding antibodies and their validation
  • How to apply modern validation standards for IHC antibodies
  • The International Working Group for Antibody Validation (IWGAV) and the pillars of antibody validation
  • Challenges in mIF
  • Optimization of mIF assays

Who may this interest?

  • Translational and clinical researchers studying immuno-oncology
  • Researchers studying the tumor microenvironment
  • Researchers interested in advanced imaging techniques
  • Spatial biology researchers
  • Researchers studying immune checkpoint inhibitors


Mike Spencer, PhD
Director of Immunohistochemistry and Digital Pathology
Fortis Life Sciences (TX, USA)

Mike Spencer is Director of Immunohistochemistry and Digital Pathology at Fortis Life Sciences. He received his doctorate in Molecular and Cellular Biochemistry from the University of Kentucky. The immunohistochemistry lab at Fortis Life Sciences validates the performance of new Bethyl antibody products in immunohistochemistry and immunocytochemistry applications. Dr Spencer has been integral to extending the capabilities of immunochemistry labs to include multiplex immunoassays for spatial biology.

In association with: