CRISPR-based multiplexed genome editing for improved heterologous protein engineering and expression in E. coli
What will you learn?
- How to develop and analyze site saturation mutagenesis libraries for heterologous-enzyme engineering to improve activity
- How to improve expression of heterologous proteins in E. coli using genome-wide editing libraries
- Novel promoter and ribosome binding site biochemistry for improving the understanding of genome regulation
- How to apply fluorescence-based assays for high-throughput novel variant discovery and investigations into genomic regulatory elements
Who may this interest?
- Pharmaceutical companies with protein expression workflow in their pipeline
- Structural biologists
- Synthetic biologists
Tyson Shepherd, PhD
Senior Scientist, Applications Development
Inscripta (CO, USA)
Dr Tyson Shepherd has a background in biochemistry and synthetic biology, and has published papers on protein engineering, large DNA construct design, cell-free synthetic biology, phage biology and nucleic acid nanotechnology. Dr Shepherd works in a collaborative team within Inscripta and with external partners to design and execute projects demonstrating the many applications of the Onyx Digital Genome Engineering Platform. Before joining Inscripta, Dr Shepherd was a research associate at MIT. Dr Shepherd earned his graduate degree at the University of Iowa and completed his postdoctoral research at MIT and Uppsala University.