New four-wavelength LED illumination systems bring sophisticated control to fluorescence microscopy

Written by CoolLED

CoolLED (Andover, UK) has launched its new pE-400 Series Illumination Systems where sophisticated control combines with powerful illumination for cost-effective fluorescence microscopy, from routine to advanced applications.

The pE-400 Series includes the pE-400 and pE-400max Illumination Systems, with four intense LED wavelengths offering broad spectral coverage from 365-635 nm for all common fluorophores ranging from DAPI to Cy5.

A Choice of Control

While the LED configuration is identical, the difference comes down to control. The white light pE-400 Illumination System offers a modern lamp replacement, which is ideal for applications such as fluorescence screening or sample visualisation on confocal setups, with features including:

  • Manual control pod, global BNC for TTL, and USB (Type B) for operation in third-party imaging software.
  • <10 µs TTL triggering for high temporal resolution.

For more demanding applications such as high-speed live cell imaging and optogenetics, the four-channel pE-400max also includes:

  • On/off and irradiance control of individual channels.
  • Ability to fit optional inline excitation filter holders, removing mechanical latency of filter wheels for high-speed multi-channel experiments.
  • Four-channel Sequence Runner, transforming a manual microscope into a cost-effective automated imaging system by triggering illumination sequences with a camera’s TTL output.

Win-Win for Sustainability and Performance

Supporting laboratories in achieving their sustainability goals, the pE-400 Series eliminates toxic mercury, cutting energy consumption and utilising 80% recycled aluminium. Stable and robust performance with near-silent operation come as standard, and liquid light guide or direct fit delivery ensure compatibility with most major microscope models. The Illumination Systems are also supported by CoolLED’s world-renowned customer service and a 36-month warranty.

 

About CoolLED

CoolLED designs and manufactures cutting edge LED Illumination Systems for researchers and clinicians using the latest LED technology. Since our team of four introduced the first commercially available LED Illumination System in 2006, we have led the way in transforming LED microscope lighting for fluorescence microscopy and transmitted illumination. Now we are a fast-growing company in Hampshire, UK, with a vast product range and technical expertise spanning optical engineering and the life sciences.

www.coolled.com